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of the pertinent published literature that is available on the subject. The key is to summarize (for the reader) what we knew about the specific problem before you did your experiments or studies. This is accomplished with a general review of the primary research literature (with citations) but should not include very specific, lengthy explanations that you will probably discuss in greater detail later in the Discussion. The judgment of what is general or specific is difficult at first, but with practice and reading of the scientific literature you will develop e firmer sense of your audience. In the mouse behavior paper, for example, you would begin the Introduction at the level of matingbehavior in general, then quickly focus to mouse mating behaviors and then hormonal regulation of behavior. Lead the reader to your statement of purpose/hypothesis by focusing your literature review from the more general context (the big picture e.g., hormonal modulation of behaviors) to the more specific topic of interest to you (e.g., role/effects of reproductive hormones, especially estrogen, in modulating specific sexual behaviors of mice.) Top of page What literature should you look for in your review of what we know about the problem? Focus your efforts on the primary research journals - the journals that publish original research articles. Although you may read some general background references (encyclopedias, textbooks, lab manuals, style manuals, etc.) to get yourself acquianted with the subject area, do not cite these, becasue they contain information that is considered fundamental or "common" knowledge wqithin the discipline. Cite, instead, articles that reported specific results relevant to your study. Learn, as soon as possible, how to find the primary literature (research journals) and review articles rather than depending on reference books. The articles listed in the Literature Cited of relevant papers you find are a good starting point to move backwards in a line of inquiry. Most academic libraries support the Citation Index - an index which is useful for tracking a line of inquiry forward in time. Some of the newer search engines will actually send you alerts of new papers that cite particular articles of interest to you. Review articles are particularly useful because they summarize all the research done on a narrow subject area over a brief period of time (a year to a few years in most cases). Top of page Be sure to clearly state the purpose and /or hypothesis that you investigated. When you are first learning to write in this format it is okay, and actually preferable, to use a pat statement like, "The purpose of this study was to...." or "We investigated three possible mechanisms to explain the ... (1) blah, blah..(2) etc. It is most usual to place the statement of purpose near the end of the Introduction, often as the topic sentence of the final paragraph. It is not necessary (or even desirable) to use the words "hypothesis" or "null hypothesis", since these are usually implicit if you clearly state your purpose and expectations. Top of page Provide a clear statement of the rationale for your approach to the problem studied. For example: State briefly how you approached the problem (e.g., you studied oxidative respiration pathways in isolated mitochondria of cauliflower). This will usually follow your statement of purpose in the last troduction. Why did you choose this kind of experiment or experimental design? What ar