英语论文网

(Spaethe, Tautz et al. 2001). Spectral purity of the stimulus was calculated by the percentage distance of the stimulus in relation to the end of the spectrum locus (Chittka and Wells 2004). Chromatic contrast was calculated as the distance of a colour stimulus from the centre of the colour hexagon relative to the background. Chromatic contrast is important as perception can be affected by background colour (Lunau, Wacht et al. 1996). Green receptor contrast was measured as the green receptor excitation from a stimulus relative to the background (Giurfa, Núñez et al. 1995). This contrast is relevant as green receptors and green contrast are known to affect motion in bees (Srinivasan, Lehrer et al. 1987).

Statistical analyses

The impact of wavelength on number of landings by Trigona carbonaria was investigated using a single factor analysis of variance (ANOVA) and a post hoc Tukeys HSD test (α=0.05) (Quinn and Keough 2002) using the number of landings as the dependent variable and wavelength of stimuli as the independent variable. Brightness, purity (saturation), chromatic contrast to the background and green receptor contrast of stimuli were analysed using the Spearman's rank correlation test against choices. Statistical analyses were conducted using R statistical and graphical environment (R Development Core Team, 2007). Statistical significance was set to P≤0.05.

Part 3. Does a food deceptive orchid (Caladenia carnea) exploit the innate colour preferences of Trigona carbonaria?

Plant model

Caladenia carnea is a widespread species, common to eastern Australia. The orchid is highly variable in colour, ranging from pink to white. It is pollinated by Australian native bees of the Trigona species (Adams and Lawson 1993).With bright colours and fragrance, this orchid achieves pollination by food mimicry (Adams and Lawson 1993). Thus, due to the colour variation of the orchid, C. carnea is an excellent model with which to examine floral exploitation of potential pollinators. Caladenia carnea flowers were supplied by private growers from the Australasian Native Orchid Society.

Can Trigona carbonaria perceive a difference between pink and white flowers of Caladenia carnea?

Colorimetric analysis of the pink and white Caladenia carnea flowers were used to investigate whether different colours of the orchid would be perceived as similar or different to a bees’ visual system. A spectrophotometer was used to take four measurements of each flower colour (pink versus white). The actual measurements used in the analysis were an average of each colour (Dyer, Whitney et al. 2007). To predict the probability with which insect pollinators would discriminate between different flowers, these spectra were plotted as loci in a hexagon colour space (Chittka 1992) (see ‘hexagon colour space’ methods).

Choice experiments

I conducted trials testing the preferences of bees when offered a dichotomous choice between a white versus pink Caladenia carnea flower. Each trial took place inside a flight arena. Each white and pink flower used in a trial were matched for size, placed into indiviual plastic containers (diameter= 5 cm, height=5 cm) and placed in the arena with a distance of 10 cm between flower centres. Each container was cov本论文由英语论文网提供整理，提供论文代写，英语论文代写，代写论文，代写英语论文，代写留学生论文，代写英文论文，留学生论文代写相关核心关键词搜索。